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Peptide reconstitution calculator

Enter the vial, the water you added and your dose. The calculator gives the units to draw on a U-100 insulin syringe. Or enter the units you drew to see the dose they delivered.

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mL
Syringe size
Result

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How the math works

Reconstitution is dissolving a freeze-dried (lyophilised) peptide in bacteriostatic water so it can be measured and injected. The calculation has three steps.

  1. Find the concentration. Divide the peptide in the vial by the millilitres of water you added. A 5 mg vial in 2 mL of water is 2.5 mg per mL, or 2500 mcg per mL.
  2. Find the volume for your dose. Divide your dose by that concentration. A 250 mcg dose at 2500 mcg/mL is 0.1 mL.
  3. Convert the volume to syringe units. On a U-100 insulin syringe, 100 units is 1 mL, so multiply millilitres by 100. 0.1 mL is 10 units.

1 mg is 1000 mcg. A 5 mg vial holds 5000 mcg.

Worked example: the same dose with different water

Every row is a 5 mg vial and a 250 mcg dose. Only the water changes.

5 mg vial, 250 mcg dose
Water addedConcentrationUnits to draw
1 mL5000 mcg/mL5 units
2 mL2500 mcg/mL10 units
3 mL1667 mcg/mL15 units
5 mL1000 mcg/mL25 units

The dose is the same in every row. Only the volume you draw changes, and more water makes it a larger draw that is easier to read on the barrel.

U-100 syringe units and millilitres

On a U-100 syringe, 1 unit is 0.01 mL
UnitsVolume
5 units0.05 mL
10 units0.10 mL
25 units0.25 mL
50 units0.50 mL
100 units1.00 mL

Syringes hold 30 units (0.3 mL), 50 units (0.5 mL) or 100 units (1 mL). A draw larger than the barrel needs two draws or less water.

Questions people ask

How do you calculate peptide reconstitution units?

Divide the vial amount by the volume of bacteriostatic water added to get the concentration. Divide your dose by that concentration to get the volume to draw in millilitres. On a U-100 insulin syringe, multiply that volume by 100 to get units, because 100 units equals 1 mL.

How many units is 250 mcg from a 5 mg vial mixed with 2 mL?

A 5 mg vial in 2 mL of water gives 2500 mcg per mL. A 250 mcg dose is 0.1 mL, which is 10 units on a U-100 insulin syringe.

Does adding more bacteriostatic water change the dose?

No. The vial holds the same total amount of peptide however much water you add. More water spreads it across a larger volume, so you draw more units to get the same dose. Some people use more water when the dose is very small, because a larger draw is easier to measure.

What does 100 units mean on an insulin syringe?

On a standard U-100 insulin syringe, 100 units is 1 mL. So 50 units is 0.5 mL and 10 units is 0.1 mL. The unit markings measure volume, not the amount of peptide.

How do you convert mg to mcg?

1 mg is 1000 mcg. Multiply milligrams by 1000 to get micrograms, or divide micrograms by 1000 to get milligrams. A 5 mg vial holds 5000 mcg.

What is bacteriostatic water, and can sterile water be used instead?

Bacteriostatic water contains a small amount of benzyl alcohol, which lets a vial be entered more than once over a period of time. Plain sterile water has no preservative, so it is generally used for single-use situations. The calculation is the same either way, because the type of water does not change the math.

What if the draw is more than the syringe holds?

Either split it across two draws, or reconstitute with less water next time so the same dose sits in a smaller volume. The calculator flags this when it happens.

This calculator does arithmetic on the numbers you enter. It does not know what is appropriate for you, and it is not medical advice, a dosing recommendation or a substitute for a qualified healthcare professional. Many peptides are not approved for human use, and legal status varies by country. Check your own numbers independently before drawing anything.